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KEY RESOURCES TABLE

Journal: Cell stem cell

Article Title: Phase I Trial: Cirmtuzumab Inhibits ROR1 Signaling and Stemness Signatures in Patients with Chronic Lymphocytic Leukemia

doi: 10.1016/j.stem.2018.05.018

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ Reagent or Resource Source Identifier Antibodies Mouse Anti-human IgG1 Fc-HRP Southern Biotech Cat#9054–05; RRID: AB_2687484 4A5, Alexa 647 conjugated In house N/A IgG2b, A647 conjugated isotype control mAb BD Biosciences Cat#557903; RRID: AB_396928 Rabbit Anti-HS1 (human specific) Cell Signaling Technology Cat#4503S; RRID: AB_2096977 Rabbit polyclonal Anti-phospho HS1 (Y378) OriGene Cat#TA314001 Mouse monoclonal anti-RhoA Cytoskeleton Cat#ARH04 Anti-mouse IgG, HRP-linked Cell Signaling Technology Cat#7076; RRID: AB_330924 Anti-rabbit IgG, HRP-linked Cell Signaling Technology Cat#7074; RRID: AB_2099233 Human IgG Control Ab Sigma Cat#I5154; RRID: AB_1163610 Chemicals, Peptides, and Recombinant Proteins ROR1-extracellular domain In house N/A Rhotekin-RBD beads Cytoskeleton Cat#RT02 Quantum MESF Kit, Alexa Fluor 647 Bangs Laboratories Cat#555p Deposited Data Raw and analyzed RNA-seq data NCBI GEO GEO: {"type":"entrez-geo","attrs":{"text":"GSE114382","term_id":"114382"}} GSE114382 Software and Algorithms Gel-Pro Analyzer 4.0 software Media Cybernetics http://www.mediacy.com FlowJo (v.2.7.4) FlowJo https://www.flowjo.com Gene set enrichment analysis Broad Institute http://software.broadinstitute.org/gsea/index.jsp QuickCal v.2.3 analysis template Bangs Laboratories https://www.bangslabs.com/ Graphpad Prism v.6.0 GraphPad https://www.graphpad.com/scientific-software/prism/ Other TMB microwell peroxidase substrate kit SeraCare Cat#5120–0053 BSA Fisher Cat#BP1600 ELISA high-protein binding plates Costar Cat#3690 WNT5A ELISA kit Aviva Systems Biology Cat#OKEH00723, RRID: SCR_013560 Cirmtuzumab Pacific GMP/Abzena N/A Open in a separate window KEY RESOURCES TABLE Cirmtuzumab targets ROR1 signaling on CLL and reduces activation of RhoA and HS1 26 patients received cirmtuzumab (up to 20 mg/kg) without dose-limiting toxicity Cirmtuzumab inhibits expression of stemness gene expression signature in CLL 4 biweekly infusions of cirmtuzumab prolonged time to next treatment (TTNT)

Techniques: Control, Recombinant, Software, Enzyme-linked Immunosorbent Assay, Binding Assay